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jc 1 working solution  (Beyotime)


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    Structured Review

    Beyotime jc 1 working solution
    Jc 1 Working Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 15008 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jc+1+working+solution/JC-1/pmc12969342-111-25-28
    Average 99 stars, based on 15008 article reviews
    jc 1 working solution - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Inhibition of Microglial TRPV1 Ameliorates Brain Injury After Intracerebral Hemorrhage by Suppressing AMPK / PINK1 ‐Mediated Mitophagy
    Article Snippet: TRPV1 siRNAs (Table ) were transfected into BV2 cells via Lipofectamine 3000 transfection reagent (Invitrogen). .. HT22 cells were stained with JC‐1 working solution (Beyotime Biotechnology, C2003S) at 37°C for 20 min to assess mitochondrial membrane potential, following the kit protocol. ..

    Article Title: Ginseng stem and leaf saponins attenuates pulmonary fibrosis by regulating TFAM-mtDNA homeostasis and suppressing ZBP1-mediated PANoptosis.
    Article Snippet: .. Mitochondria were stained using pre-warmed serum-free medium containing MitoTracker (150 nM, C1048, Beyotime) at 37 ◦C for 30 min, or JC-1 working solution for 30 min. Hoechst 33342 (C1028, Beyotime) was added when necessary and incubated for 10 min. After gentle PBS washing, a fluorescence microscope was used to observe mitochondrial morphology and MMP changes. ..

    Membrane:

    Article Title: Inhibition of Microglial TRPV1 Ameliorates Brain Injury After Intracerebral Hemorrhage by Suppressing AMPK / PINK1 ‐Mediated Mitophagy
    Article Snippet: TRPV1 siRNAs (Table ) were transfected into BV2 cells via Lipofectamine 3000 transfection reagent (Invitrogen). .. HT22 cells were stained with JC‐1 working solution (Beyotime Biotechnology, C2003S) at 37°C for 20 min to assess mitochondrial membrane potential, following the kit protocol. ..

    Article Title: Near infrared enhanced palladium loaded siraitia grosvenorii carbon dots amplify mitophagy for acute lung injury immunotherapy
    Article Snippet: .. In mitochondrial levels, to investigate the mitochondrial membrane potential (MMP), the treated cells were rinsed with PBS for 3 times, and added with 5 μg/mL JC-1 working solution (Beyotime, China). ..

    Incubation:

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: .. After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ), cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h, washed twice with PBS, and incubated with JC-1 working solution (Beyotime, C2006) at 37 °C for 30 min in the dark. .. Following three PBS washes, red (aggregates) and green (monomers) fluorescence were acquired on a confocal microscope (Nikon Eclipse C2), and the red/green intensity ratio was used to assess mitochondrial membrane potential (ΔΨm).

    Article Title: Mitophagy Activation via the YAP/Parkin Pathway Underlies the Neuroprotective Action of Tetramethylpyrazine in Cerebral Ischemia/Reperfusion Injury.
    Article Snippet: .. After OGD 2 h/R 6 h, cells were incubated with JC-1 working solution (Beyotime Biotechnology, Shanghai, China; catalog no. C2006) at 37 ◦C for 15 min. .. Cells were then centrifuged and resuspended in buffer, and immediately analyzed using a CytoFLEX S flow cytometer (Beckman Coulter, Brea, CA, USA).

    Article Title: Leucovorin enhances curcumin-induced inhibition of cell viability and ferroptosis in colorectal cancer cells
    Article Snippet: Images were captured using an inverted fluorescence microscope (Motic Incorporation, Ltd.). .. To assess MMP, CRC cells were incubated with MMP Assay Kit with JC-1 (cat. no. C2006; Beyotime Institute of Biotechnology) for 24 h. The JC-1 working solution was prepared according to the manufacturer's instructions. ..

    Article Title: Ginseng stem and leaf saponins attenuates pulmonary fibrosis by regulating TFAM-mtDNA homeostasis and suppressing ZBP1-mediated PANoptosis.
    Article Snippet: .. Mitochondria were stained using pre-warmed serum-free medium containing MitoTracker (150 nM, C1048, Beyotime) at 37 ◦C for 30 min, or JC-1 working solution for 30 min. Hoechst 33342 (C1028, Beyotime) was added when necessary and incubated for 10 min. After gentle PBS washing, a fluorescence microscope was used to observe mitochondrial morphology and MMP changes. ..

    Article Title: Mitophagy Activation via the YAP/Parkin Pathway Underlies the Neuroprotective Action of Tetramethylpyrazine in Cerebral Ischemia/Reperfusion Injury
    Article Snippet: .. After OGD 2 h/R 6 h, cells were incubated with JC-1 working solution (Beyotime Biotechnology, Shanghai, China; catalog no. C2006) at 37 °C for 15 min. .. Cells were then centrifuged and resuspended in buffer, and immediately analyzed using a CytoFLEX S flow cytometer (Beckman Coulter, Brea, CA, USA).

    Mmp Assay:

    Article Title: Leucovorin enhances curcumin-induced inhibition of cell viability and ferroptosis in colorectal cancer cells
    Article Snippet: Images were captured using an inverted fluorescence microscope (Motic Incorporation, Ltd.). .. To assess MMP, CRC cells were incubated with MMP Assay Kit with JC-1 (cat. no. C2006; Beyotime Institute of Biotechnology) for 24 h. The JC-1 working solution was prepared according to the manufacturer's instructions. ..

    Gentle:

    Article Title: Ginseng stem and leaf saponins attenuates pulmonary fibrosis by regulating TFAM-mtDNA homeostasis and suppressing ZBP1-mediated PANoptosis.
    Article Snippet: .. Mitochondria were stained using pre-warmed serum-free medium containing MitoTracker (150 nM, C1048, Beyotime) at 37 ◦C for 30 min, or JC-1 working solution for 30 min. Hoechst 33342 (C1028, Beyotime) was added when necessary and incubated for 10 min. After gentle PBS washing, a fluorescence microscope was used to observe mitochondrial morphology and MMP changes. ..

    Fluorescence:

    Article Title: Ginseng stem and leaf saponins attenuates pulmonary fibrosis by regulating TFAM-mtDNA homeostasis and suppressing ZBP1-mediated PANoptosis.
    Article Snippet: .. Mitochondria were stained using pre-warmed serum-free medium containing MitoTracker (150 nM, C1048, Beyotime) at 37 ◦C for 30 min, or JC-1 working solution for 30 min. Hoechst 33342 (C1028, Beyotime) was added when necessary and incubated for 10 min. After gentle PBS washing, a fluorescence microscope was used to observe mitochondrial morphology and MMP changes. ..

    Microscopy:

    Article Title: Ginseng stem and leaf saponins attenuates pulmonary fibrosis by regulating TFAM-mtDNA homeostasis and suppressing ZBP1-mediated PANoptosis.
    Article Snippet: .. Mitochondria were stained using pre-warmed serum-free medium containing MitoTracker (150 nM, C1048, Beyotime) at 37 ◦C for 30 min, or JC-1 working solution for 30 min. Hoechst 33342 (C1028, Beyotime) was added when necessary and incubated for 10 min. After gentle PBS washing, a fluorescence microscope was used to observe mitochondrial morphology and MMP changes. ..



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    Yesen Biotech jc‒1 working solution
    ATST induces ER stress and promotes apoptosis in CRC cells A Expression of ER stress‒related proteins in HCT116 cells after ATST treatment B Changes in intracellular Ca 2+ levels in HCT116 cells C Alterations in XBP‒1s mRNA expression in HCT116 cells D Representative IF images of XBP‒1s in HCT116 cells after ATST treatment. Scale bar: 50 μm E Apoptosis rate in HCT116 cells after ATST treatment F Changes in <t>JC‒1</t> ratio in HCT116 cells following ATST treatment G Expression of apoptosis‒related proteins in HCT116 cells after ATST treatment H Representative IF images of CHOP in HCT116 cells after ATST treatment. Scale bar: 50 μm I Expression of SQSTM1 and LC3 A/B proteins in HCT116 cells after ATST treatment J Change in ROS levels in HCT116 cells K Cell cycle distribution in HCT116 cells after ATST treatment
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    Image Search Results


    ATST induces ER stress and promotes apoptosis in CRC cells A Expression of ER stress‒related proteins in HCT116 cells after ATST treatment B Changes in intracellular Ca 2+ levels in HCT116 cells C Alterations in XBP‒1s mRNA expression in HCT116 cells D Representative IF images of XBP‒1s in HCT116 cells after ATST treatment. Scale bar: 50 μm E Apoptosis rate in HCT116 cells after ATST treatment F Changes in JC‒1 ratio in HCT116 cells following ATST treatment G Expression of apoptosis‒related proteins in HCT116 cells after ATST treatment H Representative IF images of CHOP in HCT116 cells after ATST treatment. Scale bar: 50 μm I Expression of SQSTM1 and LC3 A/B proteins in HCT116 cells after ATST treatment J Change in ROS levels in HCT116 cells K Cell cycle distribution in HCT116 cells after ATST treatment

    Journal: Cell Communication and Signaling : CCS

    Article Title: Atorvastatin promotes lipid catabolism in colorectal cancer via FDFT1‒mediated inhibition of the PI3K/AKT pathway

    doi: 10.1186/s12964-026-02802-6

    Figure Lengend Snippet: ATST induces ER stress and promotes apoptosis in CRC cells A Expression of ER stress‒related proteins in HCT116 cells after ATST treatment B Changes in intracellular Ca 2+ levels in HCT116 cells C Alterations in XBP‒1s mRNA expression in HCT116 cells D Representative IF images of XBP‒1s in HCT116 cells after ATST treatment. Scale bar: 50 μm E Apoptosis rate in HCT116 cells after ATST treatment F Changes in JC‒1 ratio in HCT116 cells following ATST treatment G Expression of apoptosis‒related proteins in HCT116 cells after ATST treatment H Representative IF images of CHOP in HCT116 cells after ATST treatment. Scale bar: 50 μm I Expression of SQSTM1 and LC3 A/B proteins in HCT116 cells after ATST treatment J Change in ROS levels in HCT116 cells K Cell cycle distribution in HCT116 cells after ATST treatment

    Article Snippet: Cells were seeded in 6‒well plates at a density of 5 × 105 cells per well and treated with ATST at specified concentrations for 48 h. Cells were then collected and incubated with JC‒1 working solution (Yesen, 717164ES03, Shanghai) at 37 °C for 20 min. After washing twice with serum‒containing medium, samples were analysed by flow cytometry.

    Techniques: Expressing